It is the most abundant protein in serum. The Flex Piercing model of Sebia CZE aids in testing of human blood with capped tubes which in turn eliminates the biohazard xassociated with handling of uncapped samples.Īlbumin is a 69 kDa protein. Sebia Minicap Flex Piercing capillary electrophoresis works on the principle of capillary electroendosmosis under high-voltage electric current. The use of “electroendosmosis” principle which improves the resolution of separationĮmploying a “high-voltage” electric current which aids in improving the throughput (the processing time) and the resolution of protein separation.īelow is an illustration of capillary electrophoresis (Sebia Minicap Flex Piercing) ( Figure 1). CZE provides an improved resolution due to the following factors: Capillary electrophoresis is the preferred method when compared to its competitors including agarose gel electrophoresis due to the following reasons. when a capillary medium is used, the technique is known as capillary zone electrophoresis (CZE). There are several support mediums available for separation of serum proteins including agarose, cellulose acetate, capillary medium etc. The most commonly employed variant of electrophoresis for serum protein separation is zone electrophoresis in which the serum proteins are separated into zones or fractions and interpreted accordingly. This physical property of proteins is exploited for its separation by employing the electrophoretic technique. Smaller proteins usually migrate faster, and larger proteins take a longer time. Proteins vary in their size and shape and have the charges determined by the dissociation contents of their amino acids. Some factors to mention are the strength of the electric field, temperature of the system, pH of the ions, concentration of buffer etc. The rate at which proteins move in an electric field is determined by a number of factors of the electrophoretic system and the nature of proteins itself. The separation of proteins by electrophoresis is based on the fact that charged molecules usually migrate through a matrix/medium upon application of an electrical field.
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